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Catalog Number | orb1294314 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Estrogen Receptor alpha |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / Extracellular domain corresponding to Human Estrogen Receptor alpha |
Dilution range | Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:200 Immunohistochemistry (Paraffin) 1:200 – 1:250 |
Conjugation | Unconjugated |
Target | ESR1 / Estrogen receptor alpha |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Estrogen Receptor alpha antibody at 1/800 dilution, Lysates at 60 μg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294314 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.
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