ESR1 Antibody

• Catalog Number: orb1252087
• Category: Antibodies
• Description: ESR1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: NR3Ga-1
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Estrogen Receptor alpha protein (aa 2-185) was used as the immunogen for this antibody.
• Concentration: 0.2 mg/mL
• Dilution range: WB: use antibody at 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titration of the antibody may be required for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ESR1
• UniProt ID: P03372
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, E
• Note: For research use only
• Application notes: WB: use antibody at 0.5-1 ug/mlIHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titration of the antibody may be required for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

MCF7 cells western blot tested with Estrogen receptor antibody (ER505).

IHC testing of human breast carcinoma stained with Estrogen receptor antibody (NR3Ga-1).