ESR1 Antibody

• Catalog Number: orb1251942
• Category: Antibodies
• Description: ESR1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM567
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant human Estrogen Receptor alpha protein (amino acids 2-185) was used as the immunogen for this Estrogen Receptor alpha antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the Estrogen Receptor antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ESR1
• UniProt ID: P03372
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, E
• Note: For research use only
• Application notes: Western blot: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the Estrogen Receptor antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Estrogen Receptor antibody (SPM567).

Western blot of Estrogen Receptor in MCF-7 lysate using Estrogen Receptor antibody (SPM567).