ERp57 Rabbit Polyclonal Antibody

• Catalog Number: orb183430
• Category: Antibodies
• Description: ERp57 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Gallus, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ERp57 (351-450/505aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: PDIA3
• UniProt ID: P30101
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ERp57/ERp60; 58 kDa glucose regulated protein; 58
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhu, Yanmei et al. Differential Proteomic Analysis Reveals Protein Networks and Pathways that May Contribute to Helicobacter pylori FKBP-Type PPIase-Associated Gastric Diseases Proteomics Clin Appl, 12, e1700127 (2018)

PubMed: 29148176

Sample: Lane 1: Mouse Skin tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Placenta tissue lysates, Lane 4: Rat Testis tissue lysates, Lane 5: Human A375 cell lysates, Lane 6: Human HepG2 cell lysates, Lane 7: Human HeLa cell lysates, Primary: Anti-ERp57 (orb183430) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 55 kDa.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ERp57) Polyclonal Antibody, Unconjugated (orb183430) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: embryo (Mouse) Lysate at 40 ug, Primary: Anti-ERp57 (orb183430) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.