ERK1/2 antibody

• Catalog Number: orb1474616
• Category: Antibodies
• Description: Rabbit monoclonal antibody to ERK1/2
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein of human ERK2
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: MAPK3; MAPK1
• Entrez: 116590, 26417, 26413, 50689, 5594, 5595
• UniProt ID: P63086, P21708, P27361, Q63844, P28482, P63085
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: MAPK3; ERK1; PRKM3; Mitogen-activated protein kina
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ERK1/2 expression in K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates. (Predicted band size: 44, 42 kD; Observed band size: 44, 42 kD)

Immunofluorescent analysis of ERK1/2 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.