ERK 8 rabbit pAb

• Catalog Number: orb765175
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ERK 8
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human MAPK15. AA range:141-190
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 60
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-MAPK15 antibody, anti-ERK7 antibody, anti-ERK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using ERK 8 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunofluorescence analysis of NIH/3T3 cells, using ERK8 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from HepG2 cells, using ERK8 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).