ERF Antibody

• Catalog Number: orb1527942
• Category: Antibodies
• Description: ERF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human, Mouse
• Immunogen: between 498 and 548
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. A301-702A has not performed satisfactorily when used for WB of ERF in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ERF.; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ERF
• UniProt ID: P50548
• NCBI: NP_006485.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: CHYTS; PE-2; PE2; Ets2 repressor factor; CRS4; ETS
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ERF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ERF antibody orb1527942 used for IP at 3 µg/mg lysate.

Detection of human and mouse ERF by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-ERF (Cat. No. orb1527942) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB