ERBB3 Rabbit Polyclonal Antibody

SKU: orb10373

Description

ERBB3 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Epidermal Growth Factor, G Protein Signaling Pathway, Receptor Tyrosne Kinases, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HER3 (661-760/1342aa)
• Target: ERBB3
• Molecular Weight: 147 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Receptor tyrosine-protein kinase erbB-3; ErbB-3; c erbB 3; c-erbB3; ErbB 3; cerbB3; ERBB3 protein; cerbB; 3erbB3 S; Glial growth factor receptor; HER 3; HER3; LCCS2; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; proto-oncogene-; receptor tyrosine protein kinase ERB3 [precursor]; Receptor tyrosine protein kinase erbB 3; Receptor tyrosine protein kinase erbB3; Tyrosine kinase type cell surface receptor HER3; V erb b2 avian erythroblastic leukemia viral oncogene homolog 3; v erb b2 erythroblastic leukemia viral oncogene homolog 3(avian); v erb b2 erythroblastic leukemia viral oncogene homolog 3; ERBB3_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ErbB-3) Polyclonal Antibody, Unconjugated (orb10373) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Tissue/Cell: Human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ErbB 3 Polyclonal Antibody, Unconjugated (orb10373) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ERBB3

UniProt

P21860