ERBB2 Antibody

• Catalog Number: orb1253801
• Category: Antibodies
• Description: ERBB2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: HER2 (Phospho-Tyr877) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 877 (T-E-Y (p) -H-A) derived from Human HER2.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 185 kDa
• Target: ERBB2
• UniProt ID: P04626
• NCBI: NP_001005862.1
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: NEU, NGL, HER2, TKR1, CD340, HER-2, MLN19, HER-2/n
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from A431 cells untreated or treated with EGF using HER2 (Phospho-Tyr877).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HER2 (Phospho-Tyr877). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr877).