ErbB2 Antibody

• Catalog Number: orb1274469
• Category: Antibodies
• Description: ErbB2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1160 to the C-terminus of human HER2 / ErbB2 (NP_004439.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 185kDa
• Target: ErbB2
• UniProt ID: P04626
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: erbB-2 Antibody: NEU, NGL, HER2, TKR1, CD340, HER-
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HER2/ErbB2 antibody (orb1274469) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts from normal (control) and HER2/ErbB2 knockout (KO) HeLa cells, using HER2/ErbB2 antibody (orb1274469) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Immunohistochemistry of paraffin-embedded rat lung using HER2/ErbB2 antibody (orb1274469) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using HER2/ErbB2 antibody (orb1274469) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using HER2/ErbB2 antibody (orb1274469) at dilution of 1:100 (40x lens).