ERAS Rabbit Polyclonal Antibody (FITC)

SKU: orb102544

Description

ERAS Rabbit Polyclonal Antibody (FITC)

Images & Validation

• Tested Applications: IF
• Dilution range: IF=1:100-500
• Predicted Reactivity: Bovine, Canine, Equine, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ERAS/HRAS2 (20-120/233aa)
• Target: ERAS
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

E ras; E-Ras; ecat5; Embryonic stem cell expressed Ras; Embryonic stem cell-expressed Ras; Eras; ES cell expressed Ras; GTPase ERas; Ha-Ras2; HRAS2; HRASP; MGC126691; MGC126693; OTTHUMP00000065857; RASE_HUMAN; Small GTPase protein E Ras; v Ha ras Harvey rat sarcoma viral oncogene homolog pseudogene; v Ha ras Harvey rat sarcoma viral oncogene homolog 2.

Blank control (Black line): U937 (Black). Primary Antibody (green line): Rabbit Anti-ERAS/FITC antibody (orb102544), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Mouse embryo), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-ERAS Antibody, conjugated (orb102544) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

ERAS