ERα (phospho Ser118) rabbit pAb

• Catalog Number: orb764186
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ERalpha (phospho-Ser118)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Ser118. AA range:91-140
• MW: 66kD
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-ESR1 antibody, anti-ESR antibody, anti-NR3A1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using Phospho-ERα (S118) Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of 293 cells using Phospho-ERα (S118) Polyclonal Antibody diluted at 1:2000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser118) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from MCF7 cells treated with Estradiol, using Estrogen Receptor-alpha (Phospho-Ser118) Antibody. The lane on the left is blocked with the phospho peptide.