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| Catalog Number | orb764186 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to ERalpha (phospho-Ser118) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Ser118. AA range:91-140 |
| MW | 66kD |
| Tested applications | ELISA, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-ESR1 antibody, anti-ESR antibody, anti-NR3A1 Read more... |
| Note | For research use only |
Western Blot analysis of various cells using Phospho-ERα (S118) Polyclonal Antibody diluted at 1:2000.
Western Blot analysis of 293 cells using Phospho-ERα (S118) Polyclonal Antibody diluted at 1:2000.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser118) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from MCF7 cells treated with Estradiol, using Estrogen Receptor-alpha (Phospho-Ser118) Antibody. The lane on the left is blocked with the phospho peptide.
