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Epsin 1 Rabbit Polyclonal Antibody

Catalog Number: orb156765

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb156765
CategoryAntibodies
DescriptionEpsin 1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsICC, IF, IHC-Fr, IHC-P
Predicted ReactivityCanine, Equine, Mouse, Sheep
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Epsin 1/EPN1 (101-200/576aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeIHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW60 kDa
TargetEPN1
UniProt IDQ9Y6I3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesEH domain binding mitotic phosphoprotein; EPN1; EP
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NoteFor research use only
Expiration Date12 months from date of receipt.
Epsin 1 Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Epsin 1) polyclonal Antibody, Unconjugated (orb156765) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Epsin 1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Epsin 1 Rabbit pAb) Polyclonal Antibody, Unconjugated (orb156765) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Epsin 1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Epsin 1 Rabbit pAb) Polyclonal Antibody, Unconjugated (orb156765) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Epsin 1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Epsin 1 Rabbit pAb) Polyclonal Antibody, Unconjugated (orb156765) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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    Human, Mouse, Rat

    Rabbit

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    200 μl, 100 μl, 50 μl
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    Mouse

    Human

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