EPHB1 Antibody

• Catalog Number: orb1263085
• Category: Antibodies
• Description: EPHB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This EphB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 955-984 amino acids from the C-terminal region of human EphB1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 110 kDa
• Target: EPHB1
• UniProt ID: P54762
• NCBI: P54762
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Ephrin type-B receptor 1, ELK, EPH tyrosine kinase
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from A431, Hela, MDA-MB-453 cell line (from left to right), using EPHB1 Antibody (H970) at 1:1000 at each lane.

Western blot analysis of anti-EphB1 Pab in mouse brain tissue

Immunohistochemical analysis of EphB1 in gastric cancer tissues. a EphB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment. b EphB1 protein focally positively stained in well-differentiated gastric cancer cells. c EphB1 protein is focally positive in poorly differentiated gastric cancer cells. d Loss of EphB1 expression in gastric cancer cells. (Provided by Jian-dong Wang, Department of Pathology Nanjing Jinling Hospital/Nanjing University School of Medicine)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis in T47D cell line lysates (35 ug/lane).