EPHB1/2 (phospho-Y594/604) antibody

• Catalog Number: orb224166
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EPHB1/2 (Phospho-Y594/604)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y594/604 of human EPHB1/2 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IF/ICC: 1:100-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EPHB1; EPHB2
• Entrez: 24338, 270190, 2047, 1969
• UniProt ID: P29323, P54762, Q8CBF3, P09759, P54763
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti EPHB1 antibody, anti ELK antibody, anti EPHT2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EPHB1/2 (Phospho-Y594/604) expression in A431 (A), HeLa (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 109; 117 kD; Observed band size: 130 kD)

Immunofluorescent analysis of EPHB1/2 (Phospho-Y594/604) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Direct ELISA antibody dose-response curve using Anti-EPHB1/2 (Phospho-Y594/604) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.