EphB1 Antibody (C-term)

SKU: orb1929083

Description

EphB1 Antibody (C-term)

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:100-500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 109885 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ELK, EPHT2, HEK6, NET

EPHB1 Antibody (H970) western blot analysis in T47D cell line lysates (35 ug/lane).This demonstrates the EPHB1 antibody detected the EPHB1 protein (arrow).

Western blot analysis of anti-EphB1 Pab in mouse brain tissue. EphB1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of lysates from A431, Hela, MDA-MB-453 cell line (from left to right), using EPHB1 Antibody (H970). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunohistochemical analysis of EphB1 in gastric cancer tissues. a) EphB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment. b) EphB1 protein focally positively stained in well-differentiated gastric cancer cells. c) EphB1 protein is focally positive in poorly differentiated gastric cancer cells. d) Loss of EphB1 expression in. gastric cancer cells.

Quick Database Links

UniProt

P54762