EphA2 Rabbit Polyclonal Antibody

• Catalog Number: orb10598
• Category: Antibodies
• Description: EphA2 Rabbit Polyclonal Antibody
• Target: EPHA2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Human, Monkey, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human EphA2 (901-976/976aa)
• UniProt ID: P29317
• RRID: AB_10751539
• MW: 105 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ARCC2; Eck; Eph receptor A2 antibody EphA2; Ephrin
• Research Area: Axonal Guidance Proteins, Cell Biology, Eph Eecept
• Note: For research use only

Sample: Human colon carcinoma lysates Lysate at 30 ug, Intestine (Mouse) Lysate at 30 ug, Primary: Anti-EphA2/Eph receptor A2 (orb10598) at 1/200 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/3000 dilution, Predicted band size: 105kD, Observed band size: 105kD, 52/55kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-EphA2 (orb10598) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 105 kD, Observed band size: 115 kD.

Tissue/Cell: mouse intestine tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-EphA2/Eph receptor A2 Polyclonal Antibody, Unconjugated (orb10598) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.