EphA2 antibody

• Catalog Number: orb1428723
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EphA2.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB01579
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 108266
• Target: EPHA2
• UniProt ID: P29317
• NCBI: NP_004422.2
• Alternative names: Ephrin type-A receptor 2, Epithelial cell kinase,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot:Anti-EPHA2 Antibody (T45) at 1:1000 dilution + MCF-7.

Western blot analysis of hEPHA2-T45 in MCF-7 cell line lysates. EPHA2 (arrow) was detected using the purified Pab.

Western blot analysis of hEPHA2-T45 in mouse NIH-3T3 cell line lysates.EPHA2 (arrow) was detected using the purified Pab.

Western blot analysis of EPHA2 (arrow) using rabbit polyclonal hEPHA2-T45.293 cell lysates either nontransfected (Lane 1) or transiently transfected with the EPHA2 gene (Lane 2).

Immunohistochemical analysis of human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Flow cytometric analysis of NCI-H292 cells using EphA2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.