EpCAM Antibody

• Catalog Number: orb640035
• Category: Antibodies
• Description: EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: EGP40/2571R
• Tested applications: FACS, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A recombinant human partial protein (amino acids 100-224) was used as the immunogen for the EpCAM antibody. The epitope of this mAb has been mapped to between amino acids 202-212.
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant EpCAM antibody is available for research use only.
• UniProt ID: P16422
• Storage: Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of human MCF7 cells with recombinant EpCAM antibody (clone EGP40/2571R); Red=isotype control, Blue= recombinant EpCAM antibody.

IHC staining of FFPE human prostate carcinoma with recombinant EpCAM antibody (clone EGP40/2571R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma with recombinant EpCAM antibody (clone EGP40/2571R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2571R) as confirmation of integrity and purity.