EpCAM Antibody / Cytoplasmic domain

• Catalog Number: orb2637307
• Category: Antibodies
• Description: Epithelial cellular adhesion molecule (Ep-CAM), also called epithelial specific antigen (ESA), is a 40-43kDa transmembrane epithelial glycoprotein. It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A affinity
• Immunogen: A portion of amino acids 289-314 of human EpCAM was used as the immunogen for the recombinant EpCAM antibody.
• UniProt ID: P16422
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: EGP40/7035R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant EpCAM antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human colon tissue with recombinant EpCAM antibody (clone EGP40/7035R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human brain tissue with recombinant EpCAM antibody (clone EGP40/7035R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (EGP40/7035R) as confirmation of integrity and purity.