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EP300 Rabbit Polyclonal Antibody

Catalog Number: orb156743

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb156743
CategoryAntibodies
DescriptionEP300 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Porcine, Rat, Sheep
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human EP300 (1451-1600/2414aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW264 kDa
TargetEP300
UniProt IDQ09472
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesEP300_HUMAN; Histone acetyltransferase p300; EC:2.
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NoteFor research use only
Expiration Date12 months from date of receipt.
EP300 Rabbit Polyclonal Antibody

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-Ep300 antibody (orb156743), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

EP300 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EP300) Polyclonal Antibody, Unconjugated (orb156743) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

EP300 Rabbit Polyclonal Antibody

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Ep300 (orb156743) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 264 kD, Observed band size: 264 kD.

EP300 Rabbit Polyclonal Antibody

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-Ep300 (orb156743) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 264 kD, Observed band size: 264 kD.

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