ENPP1 antibody

• Catalog Number: orb19086
• Category: Antibodies
• Description: Goat polyclonal antibody to ENPP1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, IHC-P: 8 μg/ml
• Conjugation: Unconjugated
• MW: 105
• Target: ENPP1 / PC1
• Entrez: 5167
• Protein Sequence: KTHLPTFSQED
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ENPP1 antibody, anti PC-1 antibody, anti ecto
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Liver shows membranous staining in many hepatocytes. This antibody has also been successfully used on Human Pancreas showing membranous staining of acinar cells. Recommended concentration, 1-2ug/ml.WB: In transfected HEK293 transiently expressing ENPP1 a band of approx. 100 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 105kDa according to NP_006199.2. Recommended concentration, 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19086 Negative Control showing staining of paraffin embedded Human Thyroid Gland, with no primary antibody.

orb19086 (4µg/ml) staining of paraffin embedded Human Thyroid Gland. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

orb19086 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.