Enolase-2 Rabbit Polyclonal Antibody

SKU: orb1735065

Description

Enolase-2 Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:2000– 1:14000, Immunofluorescence 1:500 – 1:1000, Immunohistochemistry (Paraffin) 1:400 – 1:600
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide /Enolase-2 / ENO2
• Target: Enolase-2 / ENO2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-NSE / Enolase-2 antibody at 1/3000 dilution, Lysates at 30 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane was then blocked for an hour before being incubated with orb1735065 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded human brain tissue labeling NSE / Enolase-2 with orb1735065 at 1/400.

Immunohistochemical analysis of paraffin embedded human brain tissue labeling NSE / Enolase-2 with orb1735065 at 1/400.

Immunohistochemical analysis of paraffin embedded human brain tissue labeling NSE / Enolase-2 with orb1735065 at 1/400.

Immunohistochemical analysis of paraffin embedded human brain tissue labeling NSE / Enolase-2 with orb1735065 at 1/400.

Immunohistochemical analysis of paraffin embedded Human hepatocellular carcinoma tissue labeling NSE / Enolase-2 with orb1735065 at 1/400.

Immunohistochemical analysis of paraffin embedded Human lung cancer tissue labeling NSE / Enolase-2 with orb1735065 at 1/400.

Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling NSE / Enolase-2 with orb1735065 at 1/400.

Immunohistochemical analysis of paraffin embedded Human prostate cancer tissue labeling NSE / Enolase-2 with orb1735065 at 1/400.

Immunohistochemical analysis of paraffin embedded Human stomach cancer tissue labeling NSE / Enolase-2 with orb1735065 at 1/400.

Immunohistochemical analysis of paraffin embedded Human Tonsillar malignancy tissue labeling NSE / Enolase-2 with orb1735065 at 1/400.

Immunohistochemistry (Frozen sections), Sample: mouse retina tissue sections, Antibody: Enolase-2 / ENO2 antibody (orb1735065) at 1/500 dilution.

Quick Database Links

Gene Symbol

Enolase-2 / ENO2