ENO1 Rabbit Polyclonal Antibody

SKU: orb3114780

Description

The ENO1 Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in FC, ICC, IF, IHC, IP, and WB applications. This antibody targets the Alpha-enolase in Human, Monkey, Mouse, and Rat samples.

Research Area

Cardiovascular Research, Cell Biology, Disease Biomarkers, Hematology, Immunology & Inflammation, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Dilution Range: Western blot, 0.1-0.25μg/ml, Human, Mouse, Monkey, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human, Monkey, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ENO1, which shares 90.9% amino acid (aa) sequence identity with rat ENO1.
• Target: Alpha-enolase
• Molecular Weight: 47 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Alpha enolase; C myc promoter binding protein; ENO1; ENO1L1; Enolase 1; enolase 1; (alpha); MBP 1; MBPB1; MPB 1; MPB1; NNE; Non neural enolase; Phosphopyruvate hydratase; Plasminogen binding protein; PPH

Quick Database Links

Gene Symbol

Alpha-enolase

UniProt

P06733