ENO1 Antibody

• Catalog Number: orb1274554
• Category: Antibodies
• Description: ENO1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 47kDa
• Target: ENO1
• UniProt ID: P06733
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ENO1, NNE, PPH, MPB1, ENO1L1
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ENO1 antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using ENO1 antibody at 1:3000 dilution.

Immunofluorescence analysis of NIH-3T3 cells using ENO1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using ENO1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody. Western blot was performed from the immunoprecipitate using ENO1 antibody at a dilution of 1:1000.