Endoglin antibody

• Catalog Number: orb771525
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Endoglin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Synthetic peptide from human protein at AA range: 370-430
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 70
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-Endoglin (CD antigen CD105) antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from 1) 3T3, 2) K562, 3) Hela cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Monoclonal Antibody (2B8) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

Western blot analysis of 3T3 KB K562 Hela 293T lysate, antibody was diluted at 500. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200.