ELYS Rabbit Polyclonal Antibody

SKU: orb1089

Description

ELYS Rabbit Polyclonal Antibody

Research Area

Cardiovascular Research, Cell Biology, DNA and RNA Research, Transcription Factors

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Equine, Human, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ELYS (451-550/2266aa)
• Target: AHCTF1
• Molecular Weight: 252 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ahctf1; AT hook containing transcription factor 1; DKFZp434N093; ELYS; AT-hook-containing transcription factor 1; ELYS transcription factor like protein TMBS62; ELYS_HUMAN; Embryonic large molecule derived from yolk sac; MST108; MSTP108; Protein ELYS; Protein MEL-28; Putative AT-hook-containing transcription factor 1; TMBS62.

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ELYS) Polyclonal Antibody, Unconjugated (orb1089) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Spinal cord (Mouse) Lysate at 40 ug, Primary: Anti-ELYS (orb1089) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 252 kD, Observed band size: 252 kD.

Quick Database Links

Gene Symbol

AHCTF1

UniProt

Q8IZA4