Elastase Antibody

SKU: orb344823

Description

Elastase antibody

Images & Validation

• Tested Applications: ELISA, IF, Multiplex Assay, WB
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Reactivity: Mouse
• Application Notes:
  Anti-Elastase Antibody has been tested by ELISA, western blot, and immunofluorescence. This product is assayed against 1.0 ug of Elastase [Porcine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,400 is suggested for Anti-Elastase Antibody An investigator has reported that this antibody works very well on sections of mouse embryonic pancreas tissue, specifically staining developing exocrine cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Elastase [Porcine Pancreas]
• Purity: Anti-Elastase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas]. Cross reactivity was reported for mouse Elastase (mouse embryonic pancreas). Cross reactivity against Elastase from other tissues and species has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody

Double immunofluorescence microscopy of Rabbit anti-Elastase antibody and anti-Insulin antibody. Tissue: mouse embryonic gut tissue. Fixation: 1 h in 4% PFA. Antigen retrieval: not required. Primary antibody: antibodies at 10 µg/ml for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: elastase is secreted, extracellular. Staining: elastase as green fluorescent signal with insulin staining in red.

Western Blot of Rabbit anti-Elastase Antibody. Lane 1: Elastase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Elastase Antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40000 for 30 minutes at RT. Block: orb348637 for 30 minutes at RT. Predicted/Observed size: 29 kDa, 29 kDa for Elastase.

Quick Database Links

UniProt

P00772

RefSeq (Protein):NP_998988.1