CELA1 Antibody

SKU: orb344822

Description

Elastase antibody

Images & Validation

• Tested Applications: ELISA, IF, Multiplex Assay, WB
• Dilution Range: ELISA: 1:500 - 1:3000, IF: 1:100, WB: 1:500 - 1:3000
• Reactivity: Mouse
• Application Notes:
  Anti-Elastase Antibody has been tested by ELISA, western blot, and immunofluorescence. This product is assayed against 1.0 ug of Elastase [Porcine Pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,400 is suggested for Anti-Elastase Antibody An investigator has reported that this antibody works very well on sections of mouse embryonic pancreas tissue, specifically staining developing exocrine cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Elastase [Porcine Pancreas]
• Target: CELA1
• Purity: Anti-Elastase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas]. Cross reactivity was reported for mouse Elastase (mouse embryonic pancreas). Cross reactivity against Elastase from other tissues and species has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Elastase Antibody, Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody

Double immunofluorescence microscopy of Rabbit anti-Elastase antibody and anti-Insulin antibody. Tissue: mouse embryonic gut tissue. Fixation: 1 h in 4% PFA. Antigen retrieval: not required. Primary antibody: antibodies at 10 µg/ml for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: elastase is secreted, extracellular. Staining: elastase as green fluorescent signal with insulin staining in red.

Western Blot of Rabbit anti-Elastase Antibody. Lane 1: Elastase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Elastase Antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40000 for 30 minutes at RT. Block: orb348637 for 30 minutes at RT. Predicted/Observed size: 29 kDa, 29 kDa for Elastase.

Quick Database Links

Gene Symbol

CELA1

UniProt

P00772

RefSeq (Protein):NP_998988.1