EIF4EBP1 antibody

• Catalog Number: orb216096
• Category: Antibodies
• Description: Rabbit polyclonal antibody to 4EBP1 (Phospho-T46)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T46 of human 4EBP1 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EIF4EBP1
• Entrez: 116636, 1978, 13685
• UniProt ID: Q13541, Q62622, Q60876
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Eukaryotic translation initiation factor 4E-b
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of 4EBP1 (Phospho-T46) expression in K562 PMA-treated (A), NIH3T3 PMA-treated (B), PC12 PMA-treated (C) whole cell lysates. (Predicted band size: 12 kD; Observed band size: 15 kD)

Immunohistochemical analysis of 4EBP1 (Phospho-T46) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.