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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−
Item 1 of 3
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1: 500-1: 1000, IHC-P: 1: 50-1: 200, IF: 1: 100-1: 200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Peptide sequence around aa. 207~211 (S-G-S-T-T) derived from Human eIF4E. |
| Target | EIF4E |
| Purification | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Buffer/Preservatives | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-mRNA cap-binding protein antibody, anti-eIF-4F 25 kDa subunit antibody
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Quick Database Links
Gene Symbol
EIF4E
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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