EIF3H Antibody

• Catalog Number: orb1260444
• Category: Antibodies
• Description: EIF3H Antibody
• Target: EIF3H
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1).
• UniProt ID: O15372
• MW: Observed: 40kDa
• Tested applications: IF, IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EIF3H, EIF3S3, MGC102958, eIF3-gamma, eIF3-p40
• Note: For research use only

Western blot analysis of extracts of various cell lines, using EIF3H antibody (orb1260444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of MCF7 cells using EIF3H antibody (orb1260444). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of Jurkat cells using 1 ug EIF3H antibody (orb1260444). Western blot was performed from the immunoprecipitate using EIF3H antibody (orb1260444) at a dilution of 1:1000.