EIF1AY Rabbit Polyclonal Antibody

SKU: orb183282

Description

EIF1AY Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human EIF1AY (21-120/144aa)
• Target: EIF1AY
• Molecular Weight: 16 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

eIF 1A Y isoform; eIF 4C; eIF-1A Y isoform; EIF-4C; EIF1AY; Eukaryotic translation initiation factor 1A; Eukaryotic translation initiation factor 1A, Y chromosomal; Eukaryotic translation initiation factor 1A, Y linked; Eukaryotic translation initiation factor 4C; IF1AY_HUMAN; Y-chromosomal.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EIF1AY) Polyclonal Antibody, Unconjugated (orb183282) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EIF1AY) Polyclonal Antibody, Unconjugated (orb183282) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-EIF1AY (orb183282) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 16 kD, Observed band size: 18 kD.

Quick Database Links

Gene Symbol

EIF1AY

UniProt

O14602