EGR3 Rabbit Polyclonal Antibody

SKU: orb2542

Description

EGR3 Rabbit Polyclonal Antibody

Research Area

DNA and RNA Research, Signal Transduction, Transcription Factors, Zinc Finger Protein

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human EGR3 (201-300/387aa)
• Target: EGR3
• Molecular Weight: 43 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

early growth response 3; early growth response gene 3; early growth response protein 3; early growth responsive 3; EGR-3; MGC138484; pilot; zinc finger protein pilot; EGR3_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Mouse intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EGR3) Polyclonal Antibody, Unconjugated (orb2542) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

EGR3

UniProt

Q06889