EGR1 Antibody (N-term)

• Catalog Number: orb1931351
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19179
• Tested applications: FC, IF, WB
• Predicted Reactivity: Other
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 57507 Da
• Target: This EGR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human EGR1.
• UniProt ID: P18146
• NCBI: NP_001955.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Early growth response protein 1, EGR-1, AT225, Ner
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

EGR1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of EGR1 Antibody (N-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of EGR1 Antibody (N-term) in mouse NIH-3T3 tissue lysates (35 ug/lane). EGR1 (arrow) was detected using the purified Pab.

EGR1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35 ug/lane). This demonstrates the EGR1 antibody detected the EGR1 protein (arrow).