EGR-1 Antibody

SKU: orb345477

Description

EGR-1 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:4,000 - 1:16,000, IHC: 2 µg/ml to 20 µg/ml, WB: 1:500 - 1:3,000
• Reactivity: Human, Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~58 kDa in size corresponding to EGR-1 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 85-115 of Human EGR-1.
• Purity: This affinity purified antibody is directed against human EGR-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with EGR-1.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.93 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-EGR-1 Antibody, EGR1, EGR 1, AT225 antibody, Early growth response 1 antibody, KROX24 antibody, Nerve growth factor-induced protein A antibody, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, ZNF225, Zinc finger protein 225, Zinc finger protein Krox-24

Biorbyt's Affinity Purified anti-EGR-1 antibody was used at a 10 ug/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.

Western blot using Biorbyt's Affinity Purified anti-EGR-1 antibody shows detection of a predominant band at ~58 kDa corresponding to EGR-1 present in mouse embryonic fibroblast whole cell lysate (p/n orb348722) (arrowhead). Approximately 35 µg of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.

Quick Database Links

UniProt

P18146

RefSeq (Protein):NP_001955.1