EGFR5 Rabbit Polyclonal Antibody

• Catalog Number: orb1971
• Category: Antibodies
• Description: EGFR5 Rabbit Polyclonal Antibody
• Target: CLEC14A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human EGFR5/CLEC14A (221-320/490aa)
• UniProt ID: Q86T13
• MW: 49 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: C type lectin domain family 14, member A; C14orf27
• Note: For research use only

Blank control (blue): 293T (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-EGFR5 antibody (orb1971), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: B16 Cell (Mouse) Lysate at 40 ug, DU145 Cell (Human) Lysate at 40 ug, Primary: Anti-EGFR5 (orb1971) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kD, Observed band size: 53 kD.