EGFR Recombinant Rabbit Monoclonal Antibody

SKU: orb783429

Description

EGFR Recombinant Rabbit Monoclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:2000-20000, IHC-P=1:200-500, IHC-F=1:200-500, ICC/IF=1:100-500, IF=1:200-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 3A8
• Immunogen: A synthesized peptide derived from human EGFR (1080-1110aa)
• Target: EGFR
• Molecular Weight: 130 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERBB; ERBB1; ERRP; HER1; NISBD2; NNCIS; PIG61; mENA; 9030024J15Rik; Errb1; Wa5; wa-2; wa2; ErbB-1; EGFR_HUMAN; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; 2.7.10.1; EGFR_MOUSE;

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (EGFR (3A8)) Monoclonal Antibody, Unconjugated (orb783429) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (EGFR (3A8)) Monoclonal Antibody, Unconjugated (orb783429) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (EGFR (3A8)) Monoclonal Antibody, Unconjugated (orb783429) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EGFR) Monoclonal Antibody, Unconjugated (orb783429) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (EGFR) Monoclonal Antibody, Unconjugated (orb783429) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human A549 cell lysates, Lane 2: Human U251 cell lysates, Lane 3: Human HeLa cell lysates, Primary: Anti-EGFR (orb783429) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kDa, Observed band size: 180 kDa.

Quick Database Links

Gene Symbol

EGFR

UniProt

P00533