EGFR Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1816900
• Category: Antibodies
• Description: EGFR Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 3F5
• Tested applications: ICC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human EGFR (1070-1210/1210aa)
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC/IF=1:50-1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 130 kDa
• Target: EGFR
• UniProt ID: P00533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Avian erythroblastic leukemia viral(v erb b) oncog
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human MDA-MB-231 cell lysates, Primary: Anti-EGFR (orb1816900) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kDa, Observed band size: 180 kDa.