EGFR purified MaxPab mouse polyclonal antibody (B01P)

• Catalog Number: orb2293304
• Category: Antibodies
• Description: Mouse polyclonal antibody raised against a full-length human EGFR protein.
• Clonality: Polyclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: EGFR (NP_958440.1, 1 a.a. ~ 405 a.a) full-length human protein.
• Protein Sequence: MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGLS
• Tested applications: IF, PLA, WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: NP_958440.1

Immunofluorescence of purified MaxPab antibody to EGFR on HeLa cell. [antibody concentration 10 ug/ml]

Proximity Ligation Analysis of protein-protein interactions between CRKL and EGFR. HeLa cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-EGFR mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot analysis of EGFR expression in transfected 293T cell line by EGFR MaxPab polyclonal antibody. Lane 1: EGFR transfected lysate (44.55 KDa). Lane 2: Non-transfected lysate.