EGFR antibody

• Catalog Number: orb388057
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GFR450
• Tested applications: FC, IF
• Reactivity: Human
• Isotype: Mouse / IgG2a, kappa
• Immunogen: Recombinant extracellular domain of human EGFR protein
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, IHC-P: 2-4 μg/ml
• Conjugation: Unconjugated
• MW: ~170kDa (wild type) and ~145kDa (vIII variant)
• Target: EGFR
• Entrez: 1956
• UniProt ID: P00533
• Storage: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1mg/ml.
• Alternative names: Erbb1; ERBB1; Errp; HER1; mENA; PIG61; Proto-oncog
• Note: For research use only
• Application notes: EGFR (Epidermal Growth Factor Receptor)
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of A431 cells using EGFR antibody

Immunofluorescence analysis of A431 cells using EGFR antibody

Immunofluorescence analysis of A431 cells using EGFR antibody

Immunohistochemical staining of Lung Squamous cell carcinoma tissue using EGFR antibody