EGFR antibody

• Catalog Number: orb348862
• Category: Antibodies
• Description: Human monoclonal antibody to EGFR
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: C225 (Cetuximab)
• Tested applications: Blocking, FC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: EGFR
• UniProt ID: P00533
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: EGFR, ErbB-1, HER1; epidermal growth factor recept
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Prewett, M. et al. The biologic effects of C225, a chimeric monoclonal antibody to the EGFR, on human prostate carcinoma J Immunother Emphasis Tumor Immunol, 19, 419-427 (1996)

PubMed: 9041461

Reactivity

Human

IF image of rat stomach tissue using anti-EGFR (primary antibody at 5 ug/ml)

Immunofluorescence image of rat stomach tissue using anti-EGFR (dilution at 5 ug/ml)

Immunohistochemical staining of paraffin embedded rat stomach tissue using anti-EGFR (primary antibody at 5 ug/ml)

IHC-P staining of rat lung tissue using EGFR antibody (dilution at 5 ug/ml)

IHC-P image of mouse ovary tissue using EGFR antibody (dilution of primary antibody at 5 ug/ml)

Immunohistochemical staining of paraffin embedded human breast cancer tissue using anti-EGFR (primary antibody at 5 ug/ml)

Western blot analysis of human lung cancer (lane 1), human breast cancer (lane 2), rat skin (lane 3), U251 cells (lane 4); Hels cells (lane 5) using EGFR antibody (2.5 ug/ml)