EGFR Antibody

• Catalog Number: orb180714
• Category: Antibodies
• Description: EGFR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human EGFR recombinant protein (Position: L25-K346). Human EGFR shares 89% amino acid (aa) sequence identity with mouse EGFR.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 134277 MW
• UniProt ID: P00533
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Epidermal growth factor receptor;2.7.10.1;Proto-on
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of EGFR using anti-EGFR antibody.Lane 1:HELA Cell;2:A549 Cell;3:A431 Cell.

IF analysis of EGFR using anti-EGFR antibody. EGFR was detected in immunocytochemical section of A431 cells.