EGFR Antibody

• Catalog Number: orb1530272
• Category: Antibodies
• Description: EGFR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1150 and C-term
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EGFR
• UniProt ID: P00533
• NCBI: NP_005219.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: erb-b2 receptor tyrosine kinase 1; proto-oncogene
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human EGFR by western blot.Samples: Whole cell lysate from HeLa cells. Antibody: Affinity purified rabbit anti-EGFR antibody orb1530272 at 0.04 µg/ml.

Detection of human and mouse EGFR by western blot.Samples: Whole cell lysate from HEK293T and mouse NIH 3T3 cells. Antibody: Affinity purified rabbit anti-EGFR antibody orb1530272 at 0.04 µg/ml.

Detection of human EGFR by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-EGFR used at a dilution of 1:1, 000 (1µg/ml).

Detection of human EGFR by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-EGFR antibody.