EGFR antibody

• Catalog Number: orb1474129
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human EGFR. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EGFR
• Entrez: 1956
• UniProt ID: P00533
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: ERBB; ERBB1; HER1; Epidermal growth factor recepto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EGFR expression in A431 (A), MDAMB468 (B) whole cell lysates. (Predicted band size: 134 kD; Observed band size: 175 kD)

Immunohistochemical analysis of EGFR staining in human lung adenocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of Hela cells using Anti-EGFR Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.