Cart summary

You have no items in your shopping cart.

EGFR Antibody, KO Validated

Catalog Number: orb1274540

DispatchUsually dispatched within 5-10 working days
$ 800.00
Catalog Numberorb1274540
CategoryAntibodies
DescriptionEGFR Antibody, KO Validated
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC, IP, WB
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MWObserved: 175kDa
TargetEGFR
UniProt IDP00533
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid
Alternative namesEpidermal growth factor receptor, Proto-oncogene c
Read more...
NoteFor research use only
Application notesWB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
Expiration Date12 months from date of receipt.
EGFR Antibody, KO Validated

Western blot analysis of extracts of OVCAR3 cells, using EGFR antibody (orb1274540) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

EGFR Antibody, KO Validated

Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (orb1274540) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

EGFR Antibody, KO Validated

Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody (orb1274540) at dilution of 1:100 (40x lens).

EGFR Antibody, KO Validated

Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody (orb1274540) at dilution of 1:100 (40x lens).

EGFR Antibody, KO Validated

Immunofluorescence analysis of HeLa cells using EGFR antibody (orb1274540) at dilution of 1:100. Blue: DAPI for nuclear staining.

EGFR Antibody, KO Validated

Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody (orb1274540) at dilution of 1:100. Blue: DAPI for nuclear staining.

EGFR Antibody, KO Validated

Immunofluorescence analysis of PC12 cells using EGFR antibody (orb1274540) at dilution of 1:100. Blue: DAPI for nuclear staining.

EGFR Antibody, KO Validated

Immunoprecipitation analysis of 200 ug extracts of HeLa cells using 3 ug EGFR antibody (orb1274540). Western blot was performed from the immunoprecipitate using EGFR antibody (orb1274540) at a dilution of 1:1000.

  • [KO Validated] EGFR Polyclonal Antibody [orb615464]

    IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl
  • [KO Validated] EGFR polyclonal antibody [orb1716513]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • [KO Validated] EGFR polyclonal antibody [orb1717256]

    IP,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • [KO Validated] EGFR polyclonal antibody [orb1717175]

    IF,  IHC,  IP,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl