EGF Receptor Antibody

• Catalog Number: orb653504
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human
• Dilution range: ICC: 1:200, IP: 1:500, IHC-P: 1:100
• Conjugation: Unconjugated
• UniProt ID: P00533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Avian erythroblastic leukemia viral (v erb b)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.

Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).

Immunohistochemical analysis of paraffin-embedded Lung carcinoma using EGF Receptor (3F12) mouse mAb (1/800 dilution) at the Roche Benchmark XT system.

Western blot detection of EGFR in HCC827, A549 and MDA-MB-468 cell lysates using EGFR mouse mAb (dilution 1:1000). Predicted band size: 134 Kda.Observed band size: 175KDa.