eEF2K antibody [N1N3]

• Catalog Number: orb556752
• Category: Antibodies
• Description: Rabbit polyclonal antibody to eEF2K
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human eEF-2K. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: WB: 1:500-1:10000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 82
• Target: eEF2K
• UniProt ID: O00418
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Alternative names: eukaryotic elongation factor 2 kinase , CaMKIII ,
• Note: For research use only
• Application notes: Positive control: Mouse liver , A431 , H1299 , HeLa , HepG2 , PC-12
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A431 cells using eEF2K antibody

Immunohistochemical staining of BT483 xenograft using eEF2K antibody

Western blot analysis of A431(Lane 1), H1299(Lane 2), Hela(Lane 3), Hep G2(Lane 4) using eEF2K antibody

Western blot analysis of A431(Lane 1), H1299(Lane 2), Hela(Lane 3), Hep G2(Lane 4) using eEF2K antibody

Western blot analysis of A431(Lane 1), H1299(Lane 2), Hela(Lane 3), Hep G2(Lane 4) using eEF2K antibody