EEF1E1 antibody

• Catalog Number: orb1474159
• Category: Antibodies
• Description: Mouse monoclonal antibody to EEF1E1
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IF, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant fusion protein of human EEF1E1. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/2000), IF/IC (1/10 - 1/50), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EEF1E1
• Entrez: 66143, 9521
• UniProt ID: O43324, Q9D1M4
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: AIMP3; P18; Eukaryotic translation elongation fact
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EEF1E1 expression in HepG2 (A), human testis (B), A549 (C), A431 (D), Jurkat (E), mouse brain (F) whole cell lysates. (Predicted band size: 19 kD; Observed band size: 20 kD)

Immunofluorescent analysis of EEF1E1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of Hela cells using Anti-EEF1E1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.