EEF1E1/AIMP3/p18 Antibody

• Catalog Number: orb1248347
• Category: Antibodies
• Description: EEF1E1/AIMP3/p18 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Porcine
• Immunogen: The immunogen for this antibody is: C-SKGNKYSAQGERQ
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 19kDa band observed in lysates of cell lines HepG2 and K562 (calculated MW of 19.8kDa according to NP_004271.1) and in Pig Testis lysates (calculated MW of 19.8kDa according to NP_001230474.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 19kDa
• Target: EEF1E1 / AIMP3 / p18
• UniProt ID: O43324
• NCBI: NP_001129122.1, NP_004271.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: EEF1E1, eukaryotic translation elongation factor 1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 19kDa band observed in lysates of cell lines HepG2 and K562 (calculated MW of 19.8kDa according to NP_004271.1) and in Pig Testis lysates (calculated MW of 19.8kDa according to NP_001230474.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Pancreas. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248347 (0.1 ug/ml) staining of K562 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248347 (0.3 ug/ml) staining of Pig Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248347 (5 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.